How to make a blood smear

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How To Make A Blood Smear. Use clean high-quality microscope slides. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Preparation of Blood Film. A well made blood smear consists of several areas.

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A phlebotomist a person specifically trained to draw blood first cleans and sterilizes the injection site with an antiseptic. Can occur with anemic patients Increase the angle of the slide you are using to smear with. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Clean slides with 70 to 90 alcohol and allow to dry. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species.

3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood.

Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. The slide should be clean. The blood smear is a simple blood test. Common cause of a poor blood smear. The feathered edge the monolayer and the body and base of the smear. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop.

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Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. If the blood has been refrigerated allow it to return. The feathered edge the monolayer and the body and base of the smear. This PULLS the blood across to make the smear. Clean slides with 70 to 90 alcohol and allow to dry.

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Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. Drop of blood too large or too small 2. Components of a blood smear. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Push carefully along the length of the first slide to produce a thin smear of blood.

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Drop of blood too large or too small 2. Aim for a blood droplet size of 4mm diameter. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. Components of a blood smear. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique.

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A well made blood smear consists of several areas. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide. A quick instructional video demonstrating. This PULLS the blood across to make the smear. Clean the area to be punctured with 70 alcohol.

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Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Common cause of a poor blood smear. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. Place a small drop of blood or one side about 1-2 cm from one end. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood.

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To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. The scratches allow for improved adherence of the blood film to the slide without affecting the smear morphology. Do not push the blood by having it ahead of the smearing slide. Collect blood in an EDTA tube and make the smears when back at the clinic.

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Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Parts of Blood Smear A peripheral blood film consists of 3 parts. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Put a small size of blood drop on each glass slide about half centimeter from its narrow edge on the right side.

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A quick instructional video demonstrating. How to Make a Peripheral Blood Smear. A phlebotomist a person specifically trained to draw blood first cleans and sterilizes the injection site with an antiseptic. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide.

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Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. They then tie a band above. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. The scratches allow for improved adherence of the blood film to the slide without affecting the smear morphology. Components of a blood smear.

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In infants puncture the heel. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells. The portion of blood film near the drop of blood. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques.

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In this video I quickly go over how to make a peripheral blood smear. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. A phlebotomist a person specifically trained to draw blood first cleans and sterilizes the injection site with an antiseptic. Parts of Blood Smear A peripheral blood film consists of 3 parts. Do not touch the surface of the slide where the blood smear will be made.

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This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. This PULLS the blood across to make the smear. In this video I quickly go over how to make a peripheral blood smear. The scratches allow for improved adherence of the blood film to the slide without affecting the smear morphology. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood.

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3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. In infants puncture the heel. The scratches allow for improved adherence of the blood film to the slide without affecting the smear morphology. Can occur with anemic patients Increase the angle of the slide you are using to smear with. Optimise spreading speed for length and a good feathered edge.

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The scratches allow for improved adherence of the blood film to the slide without affecting the smear morphology. Spreader slide pushed across the slide in a jerky. Do not touch the surface of the slide where the blood smear will be made. The drop and it spreads by capillary action along its edge. Do not push the blood by having it ahead of the smearing slide.

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Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells. They then tie a band above. Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions discard the first two drOps of blood from skin puncture site. Use clean high-quality microscope slides.

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Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. Now push the spreader across the slide. Can occur with anemic patients Increase the angle of the slide you are using to smear with. Blood can also be taken from an EDTA sample. Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide.

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How to perform a blood film in 3 easy stepsLearn more and visit httpswwwidexxcouk. If the blood has been refrigerated allow it to return. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide.

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Place a small drop of blood or one side about 1-2 cm from one end. The slide should be clean. Put a small size of blood drop on each glass slide about half centimeter from its narrow edge on the right side. In this video I quickly go over how to make a peripheral blood smear. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells.

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